                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology
       Moon, Tae S
       Yoon, Sang-Hwal
       Prather, Kristala L J
 
<120>  Cellular Production of Glucaric Acid

<130>  M0605.70158WO00

<140>  not yet assigned
<141>  2009-04-03

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  828
<212>  DNA
<213>  Pseudomonas syringae

<400>  1
atggcatcgg ctcataccac tcaaactccc ttcaatcgcc tcctgctgac cggtgctgca       60

ggcggcctgg gcaaggtatt gcgcgagaca ttgcgccctt actcacacat tctgcgactc      120

tctgatatcg ccgagatggc gcccgcggtc ggcgaccatg aagaagtgca ggtctgcgat      180

ctggcggaca aagacgccgt acaccgtctg gtcgaaggcg tggacgccat tctgcatttt      240

ggcggcgtgt cggtcgaacg gcctttcgag gaaatcctcg gcgctaatat ctgcggcgtg      300

ttccatatct acgaggccgc ccgccggcat ggcgtgaaac gcgtgatctt cgccagctcc      360

aaccatgtga tcggtttcta caagcagaac gaaaccatcg acgcgcactc cccgcgccgc      420

ccggacagct actatggttt gtccaagtcc tacggcgaag acatggccag cttctacttt      480

gatcgttacg gcatcgaaac cgtcagcatc cgcatcggct catcgttccc tgaaccacag      540

aaccgcagaa tgatgagcac ctggctgagc ttcgatgacc tgacccggtt gctcgagcgc      600

gccctgtaca cgccggacgt cggccacacc gtggtgtatg gcgtgtcgga caacaagacc      660

gtgtggtggg acaaccgctt tgccagcaaa ctggactacg cccctaaaga cagctcggag      720

gtcttccgcg ccaaggtcga cgcccagcca atgccggcgg acgacgaccc ggcaatggtt      780

taccagggcg gtgcgtttgt agcgtccgga ccgttcggcg ataaataa                   828


<210>  2
<211>  275
<212>  PRT
<213>  Pseudomonas syringae

<400>  2

Met Ala Ser Ala His Thr Thr Gln Thr Pro Phe Asn Arg Leu Leu Leu 
1               5                   10                  15      


Thr Gly Ala Ala Gly Gly Leu Gly Lys Val Leu Arg Glu Thr Leu Arg 
            20                  25                  30          


Pro Tyr Ser His Ile Leu Arg Leu Ser Asp Ile Ala Glu Met Ala Pro 
        35                  40                  45              


Ala Val Gly Asp His Glu Glu Val Gln Val Cys Asp Leu Ala Asp Lys 
    50                  55                  60                  


Asp Ala Val His Arg Leu Val Glu Gly Val Asp Ala Ile Leu His Phe 
65                  70                  75                  80  


Gly Gly Val Ser Val Glu Arg Pro Phe Glu Glu Ile Leu Gly Ala Asn 
                85                  90                  95      


Ile Cys Gly Val Phe His Ile Tyr Glu Ala Ala Arg Arg His Gly Val 
            100                 105                 110         


Lys Arg Val Ile Phe Ala Ser Ser Asn His Val Ile Gly Phe Tyr Lys 
        115                 120                 125             


Gln Asn Glu Thr Ile Asp Ala His Ser Pro Arg Arg Pro Asp Ser Tyr 
    130                 135                 140                 


Tyr Gly Leu Ser Lys Ser Tyr Gly Glu Asp Met Ala Ser Phe Tyr Phe 
145                 150                 155                 160 


Asp Arg Tyr Gly Ile Glu Thr Val Ser Ile Arg Ile Gly Ser Ser Phe 
                165                 170                 175     


Pro Glu Pro Gln Asn Arg Arg Met Met Ser Thr Trp Leu Ser Phe Asp 
            180                 185                 190         


Asp Leu Thr Arg Leu Leu Glu Arg Ala Leu Tyr Thr Pro Asp Val Gly 
        195                 200                 205             


His Thr Val Val Tyr Gly Val Ser Asp Asn Lys Thr Val Trp Trp Asp 
    210                 215                 220                 


Asn Arg Phe Ala Ser Lys Leu Asp Tyr Ala Pro Lys Asp Ser Ser Glu 
225                 230                 235                 240 


Val Phe Arg Ala Lys Val Asp Ala Gln Pro Met Pro Ala Asp Asp Asp 
                245                 250                 255     


Pro Ala Met Val Tyr Gln Gly Gly Ala Phe Val Ala Ser Gly Pro Phe 
            260                 265                 270         


Gly Asp Lys 
        275 


<210>  3
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  3
gaattcatga cagaagataa tattgctc                                          28


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  4
aagcttctac aacaatctct cttcg                                             25


<210>  5
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  5
aggaggtaat aaat                                                         14


<210>  6
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  6
cgaattcagg aggtacaacc atgcctgttt cag                                    33


<210>  7
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  7
cgtcgactta tcgcgcatcg gccagcagtt g                                      31


<210>  8
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  8
cgaattcagg aggattgaat catgagtc                                          28


<210>  9
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  9
cgtcgactta aagatccagc agccagc                                           27


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  10
gccatggcat cggctcatac cac                                               23


<210>  11
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  11
cgagctctta tttatcgccg aacggtcc                                          28


<210>  12
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  12
ctagaattca tgaaacggct tcttgttacc                                        30


<210>  13
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  13
ctagagctct tagctctgtt tgaagatcgg gttg                                   34


<210>  14
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  14
gtcgaattca tgaccactac ccccttcaat c                                      31


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  15
ctagagctcc gtggggttag ttgaacgggc                                        30


<210>  16
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  16
ctagaattca tggcatcggc tcataccact c                                      31


<210>  17
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  17
tcagagctcg aaacggcttc ttgttaccgg tgc                                    33


<210>  18
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  18
ctgaagcttg ctctgtttga agatcgggtt gtcg                                   34


<210>  19
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  19
tcagagctcg accactaccc ccttcaatcg cc                                     32


<210>  20
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  20
ctgaagcttg ttgaacgggc cggccacggc g                                      31


<210>  21
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  21
tcagagctcg gcatcggctc ataccactca aactcc                                 36


<210>  22
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  22
ctgaagcttt ttatcgccga acggtccgga cgc                                    33


<210>  23
<211>  798
<212>  DNA
<213>  Agrobacterium tumefaciens

<400>  23
atgaaacggc ttcttgttac cggtgcggcg ggccagcttg gccgcgtcat gcgcgagcgt       60

ctcgcaccga tggcggagat actgcgcctt gccgatctct ccccgctcga cccggcaggg      120

ccgaacgaag aatgcgtgca atgcgacctt gccgatgcca atgccgtgaa tgccatggtc      180

gccggttgcg acggtattgt tcatctcggc ggcatctcgg tggagaagcc cttcgaacaa      240

atccttcagg gcaatatcat cgggctttat aatctctacg aggccgcccg cgcccatgga      300

cagccacgca tcgtctttgc cagctccaac cacacgatcg gctattatcc gcagaccgaa      360

cggctcggtc cggatgttcc ggcgcggccg gacggtcttt acggcgtctc caaatgtttc      420

ggcgaaaacc tcgcccgcat gtatttcgat aaattcgggc aggagacggc gctggtgcgc      480

atcggctcct gtacgccgga acccaacaat taccgcatgc tgtccacctg gttttcgcac      540

gatgatttcg tgtcgctgat cgaggcggtg tttcgcgcgc cggtgctcgg ctgcccggtc      600

gtctgggggg catcggccaa tgatgcgggc tggtgggaca attcgcatct tggctttctg      660

ggctggaaac cgaaggataa tgccgaggcc ttccggcggc atataaccga gacgacaccg      720

ccaccggacc cgaatgacgc gttggtgcgg ttccagggcg gtacgtttgt cgacaacccg      780

atcttcaaac agagctga                                                    798


<210>  24
<211>  265
<212>  PRT
<213>  Agrobacterium tumefaciens

<400>  24

Met Lys Arg Leu Leu Val Thr Gly Ala Ala Gly Gln Leu Gly Arg Val 
1               5                   10                  15      


Met Arg Glu Arg Leu Ala Pro Met Ala Glu Ile Leu Arg Leu Ala Asp 
            20                  25                  30          


Leu Ser Pro Leu Asp Pro Ala Gly Pro Asn Glu Glu Cys Val Gln Cys 
        35                  40                  45              


Asp Leu Ala Asp Ala Asn Ala Val Asn Ala Met Val Ala Gly Cys Asp 
    50                  55                  60                  


Gly Ile Val His Leu Gly Gly Ile Ser Val Glu Lys Pro Phe Glu Gln 
65                  70                  75                  80  


Ile Leu Gln Gly Asn Ile Ile Gly Leu Tyr Asn Leu Tyr Glu Ala Ala 
                85                  90                  95      


Arg Ala His Gly Gln Pro Arg Ile Val Phe Ala Ser Ser Asn His Thr 
            100                 105                 110         


Ile Gly Tyr Tyr Pro Gln Thr Glu Arg Leu Gly Pro Asp Val Pro Ala 
        115                 120                 125             


Arg Pro Asp Gly Leu Tyr Gly Val Ser Lys Cys Phe Gly Glu Asn Leu 
    130                 135                 140                 


Ala Arg Met Tyr Phe Asp Lys Phe Gly Gln Glu Thr Ala Leu Val Arg 
145                 150                 155                 160 


Ile Gly Ser Cys Thr Pro Glu Pro Asn Asn Tyr Arg Met Leu Ser Thr 
                165                 170                 175     


Trp Phe Ser His Asp Asp Phe Val Ser Leu Ile Glu Ala Val Phe Arg 
            180                 185                 190         


Ala Pro Val Leu Gly Cys Pro Val Val Trp Gly Ala Ser Ala Asn Asp 
        195                 200                 205             


Ala Gly Trp Trp Asp Asn Ser His Leu Gly Phe Leu Gly Trp Lys Pro 
    210                 215                 220                 


Lys Asp Asn Ala Glu Ala Phe Arg Arg His Ile Thr Glu Thr Thr Pro 
225                 230                 235                 240 


Pro Pro Asp Pro Asn Asp Ala Leu Val Arg Phe Gln Gly Gly Thr Phe 
                245                 250                 255     


Val Asp Asn Pro Ile Phe Lys Gln Ser 
            260                 265 


<210>  25
<211>  807
<212>  DNA
<213>  Pseudomonas putida

<400>  25
atgaccacta cccccttcaa tcgcctgctg ctcaccggag ccgcaggcgg cctgggcaag       60

gtccttcgcg aacgcctgaa aggctacgcc gaggtcctgc gcctgtctga catcagcccc      120

atggccccgg ccgcgggccc gcatgaagaa gtcattacct gtgacctggc cgacaaggct      180

gcggtgcata ccctggtcga gggcgtagac gccatcatcc actttggcgg ggtttctacc      240

gaacacgcct tcgaagagat tctcggcccc aatatctgcg gcgtgttcca cgtgtacgag      300

gcggcgcgca agcacggggt caagcgcatc atcttcgcca gctccaacca caccatcggt      360

ttctatcgcc aggatgagcg catcgacgct cacgcgccgc gccggcccga cagctattac      420

gggctgtcca agtgctacgg cgaagatgtg gccagcttct actttgaccg ctacggcatc      480

gagaccgtca gcattcgcat cggctcgtcg ttcccgcagc cacagaacct gcgcatgctc      540

tgcacctggc tcagttacga cgacctggtg cagttgatcg aacgcgggct gttcaccccc      600

ggggttggcc acaccatcgt ctacggcgcc tccgacaatc gcaccgtgtg gtgggacaac      660

cgccatgccg cgcacctggg ctatgtaccc aaggacagct ccgaaacctt ccgcgcagcc      720

gtggaggccc aaccggcacc cgccgccgat gacccgagca tggtctacca gggcggcgct      780

ttcgccgtgg ccggcccgtt caactga                                          807


<210>  26
<211>  268
<212>  PRT
<213>  Pseudomonas putida

<400>  26

Met Thr Thr Thr Pro Phe Asn Arg Leu Leu Leu Thr Gly Ala Ala Gly 
1               5                   10                  15      


Gly Leu Gly Lys Val Leu Arg Glu Arg Leu Lys Gly Tyr Ala Glu Val 
            20                  25                  30          


Leu Arg Leu Ser Asp Ile Ser Pro Met Ala Pro Ala Ala Gly Pro His 
        35                  40                  45              


Glu Glu Val Ile Thr Cys Asp Leu Ala Asp Lys Ala Ala Val His Thr 
    50                  55                  60                  


Leu Val Glu Gly Val Asp Ala Ile Ile His Phe Gly Gly Val Ser Thr 
65                  70                  75                  80  


Glu His Ala Phe Glu Glu Ile Leu Gly Pro Asn Ile Cys Gly Val Phe 
                85                  90                  95      


His Val Tyr Glu Ala Ala Arg Lys His Gly Val Lys Arg Ile Ile Phe 
            100                 105                 110         


Ala Ser Ser Asn His Thr Ile Gly Phe Tyr Arg Gln Asp Glu Arg Ile 
        115                 120                 125             


Asp Ala His Ala Pro Arg Arg Pro Asp Ser Tyr Tyr Gly Leu Ser Lys 
    130                 135                 140                 


Cys Tyr Gly Glu Asp Val Ala Ser Phe Tyr Phe Asp Arg Tyr Gly Ile 
145                 150                 155                 160 


Glu Thr Val Ser Ile Arg Ile Gly Ser Ser Phe Pro Gln Pro Gln Asn 
                165                 170                 175     


Leu Arg Met Leu Cys Thr Trp Leu Ser Tyr Asp Asp Leu Val Gln Leu 
            180                 185                 190         


Ile Glu Arg Gly Leu Phe Thr Pro Gly Val Gly His Thr Ile Val Tyr 
        195                 200                 205             


Gly Ala Ser Asp Asn Arg Thr Val Trp Trp Asp Asn Arg His Ala Ala 
    210                 215                 220                 


His Leu Gly Tyr Val Pro Lys Asp Ser Ser Glu Thr Phe Arg Ala Ala 
225                 230                 235                 240 


Val Glu Ala Gln Pro Ala Pro Ala Ala Asp Asp Pro Ser Met Val Tyr 
                245                 250                 255     


Gln Gly Gly Ala Phe Ala Val Ala Gly Pro Phe Asn 
            260                 265             


